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Table 4 List of primers and their sequences that were used for PCR and genotyping

From: Genetic diversity of Aspergillus flavus and occurrence of aflatoxin contamination in stored maize across three agro-ecological zones in Kenya

Name of marker

Group no.

Primer sequence (5′–3’)

Probe (DYE)

Repeat motif and scaffold

Expected size range (base pairs)

AF8

1

F: GGCTTGCAAGTCTAATCTGC

PET

(AAG)16-2911

178

  

R: TGTGTCTTTGGGATGTATTTCG

   

AF10

1

F: CGTGCCATCGTAGAACTTCC

6-FAM

(TAC)10-2504

274

  

R: GGGACATTGGTAGTACCTTGG

   

AF11

1

F: GACGGCGGTGTACAGTGATAGT

NED

(AAG)12-2504

142

  

R: GCAGTAACGCGATTATGCAAGT

   

AF13

1

F: CGTGTTCCAAGTCAAGTCCA

VIC

(CTT)9-1866

136

  

R: TCTCCTTTGCTCCCGTTAGA

   

AF16

2

F: AGGTCGTGAAGCCGATACTG

6-FAM

(TTG)10-2541

179

  

R: CAAAGGCAGATCGAAGGGTA

   

AF48

2

F:CCACGTTCCACTGTCTCCTT

VIC

(AAG)12-2802

352

  

R: GCAAGTCCTCCACTGATGGT

   

AF54

2

F: GAGAGGTATGCCTTCATGCTTT

VIC

(ACAT)8-1918

173

  

R: AGTGTGTCGACATGGATTGC

   

AF53

3

F: TCCTCCAAAGTGACCAAAGC

6-FAM

(TCT)8-1918

147

  

R: TGCGATTGCTCAGGACATAG

   

AF55

3

F: TCATGATCAACCCAGTCCAA

PET

(GT)10-1739

169

  

R: TGGGCAGAATATCCACGTCT

   

AF64

3

F: ACTGAGCATTCACCTGCTTG

VIC

(AC)16-2856

177

  

R: ACCTAGCGGGAGGTTCTAGG

   

AF18

4

F: CCGCCTCCGAGTGTACTTA

NED

(TTC)29-1918

198

  

R: CAATAAGGATCGCAATCGTACA

   

AF25

4

F: GTGAGAGCAATTGGGAAACC

VIC

(TAC)7

304

    

TCC (TAC)-2504

 
  

R: TGACCAATATGCTGGAGGTG

   

AF43

4

F: GTGAGAGCAATTGGGAAACC

VIC

(GAG)13-2634

392

  

R: TGACCAATATGCTGGAGGTG

   
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Agriculture & Food Security

ISSN: 2048-7010