Table 2 Sequence of primers used for selective amplification
Primer pair | EcoRI fluorescent dye-labeled primers | MseI primer |
|---|---|---|
1st primer pair | 5′-GACTGCGTACCAATTCAAG-3′ | 5′-GATGAGTCCTGAGTAACAA-3′ |
2nd primer pair | 5′-GACTGCGTACCAATTCACG-3′ | 5′-GATGAGTCCTGAGTAACAG-3′ |
3rd primer pair | 5′-GACTGCGTACCAATTCAGC-3′ | 5′-GATGAGTCCTGAGTAACTG-3′ |
4th primer pair | 5′-GACTGCGTACCAATTCAGG-3′ | 5 -GATGAGTCCTGAGTAACAT-3′ |