Fig. 9

A comparison of the sequences of Botswana specimens with existing sequences on GenBank; KJ657881, KJ657680, KC852871, KT452897, KP793742, KC852872, KP324753 and an outgroup KX862248. The evolutionary history was inferred using the neighbour-joining method [53]. The optimal tree with the sum of branch length = 0.13050185 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) are shown next to the branches [54]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method [55] and are in the units of the number of base substitutions per site. The analysis involved nine nucleotide sequences. Codon positions included were first + second + third + noncoding. All positions containing gaps and missing data were eliminated. There were a total of 510 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [38]